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STEMCELL Technologies Inc stemspan cc100 cytokine cocktail
Stemspan Cc100 Cytokine Cocktail, supplied by STEMCELL Technologies Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/stemspan+cc100+cytokine+cocktail/stemspan+cc100/pm40016442-145-11-18
Average 90 stars, based on 1 article reviews
stemspan cc100 cytokine cocktail - by Bioz Stars, 2026-09
90/100 stars

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Modification:

Article Title: Anti-CLL-1 antibodies and methods of use
Article Snippet: .. Target cells were resuspended in Iscove's Modified Dulbecco's Medium (IMDM) containing the following supplements to support HSCs: 10% heat inactivated FCS, 2 mM Glutamine, StemSpan CC100 cytokine cocktail (StemCell Technologies), 20 ng/ml human GM-CSF, 20 ng/ml human G-CSF and 0.3 mg/ml low endotoxin human IgG (Molecular Innovations, Novi, Mich.) and pre-incubated for 1 h at 37° C. to block Fccustom characterRs before the addition of CD8+ T cells and TDBs. .. Serum concentration-time profiles were used to estimate PK parameters using WinNonlin software (Pharsight; Mountain View, Calif.).

Article Title: Anti-CLL-1 antibodies and methods of use
Article Snippet: .. Target cells were resuspended in Iscove's Modified Dulbecco's Medium (IMDM) containing the following supplements to support HSCs: 10% heat inactivated FCS, 2 mM Glutamine, StemSpan CC100 cytokine cocktail (StemCell Technologies), 20 ng/ml human GM-CSF, 20 ng/ml human G-CSF and 0.3 mg/ml low endotoxin human IgG (Molecular Innovations, Novi, Mich.) and pre-incubated for 1 h at 37° C. to block FcRs before the addition of CD8+ T cells and TDBs. ..

Blocking Assay:

Article Title: Anti-CLL-1 antibodies and methods of use
Article Snippet: .. Target cells were resuspended in Iscove's Modified Dulbecco's Medium (IMDM) containing the following supplements to support HSCs: 10% heat inactivated FCS, 2 mM Glutamine, StemSpan CC100 cytokine cocktail (StemCell Technologies), 20 ng/ml human GM-CSF, 20 ng/ml human G-CSF and 0.3 mg/ml low endotoxin human IgG (Molecular Innovations, Novi, Mich.) and pre-incubated for 1 h at 37° C. to block Fccustom characterRs before the addition of CD8+ T cells and TDBs. .. Serum concentration-time profiles were used to estimate PK parameters using WinNonlin software (Pharsight; Mountain View, Calif.).

Article Title: Anti-CLL-1 antibodies and methods of use
Article Snippet: .. Target cells were resuspended in Iscove's Modified Dulbecco's Medium (IMDM) containing the following supplements to support HSCs: 10% heat inactivated FCS, 2 mM Glutamine, StemSpan CC100 cytokine cocktail (StemCell Technologies), 20 ng/ml human GM-CSF, 20 ng/ml human G-CSF and 0.3 mg/ml low endotoxin human IgG (Molecular Innovations, Novi, Mich.) and pre-incubated for 1 h at 37° C. to block FcRs before the addition of CD8+ T cells and TDBs. ..

Recombinant:

Article Title: High BCR::ABL1 Expression Defines CD34+ Cells with Significant Alterations in Signal Transduction, Short-Proliferative Potential and Self-Renewal Ability
Article Snippet: Overall purity of the final cell population was >90% as verified by flow cytometer analysis using anti-CD34-PE and anti-CD45-FITC antibodies (both Beckman Coulter). .. Progenitor cells were grown in StemSpan Serum-Free Expansion medium (SFEM) supplemented, where indicated, with StemSpan CC100 Cytokine Cocktail at the following final concentrations of recombinant human (rh) cytokines: rh-Flt-3 ligand: 100 ng/mL; rh-stem cell factor: 100 ng/mL; rh interleukin-3, 20 ng/mL and rh interleukin-6, 20 ng/mL (all from Stem Cell Technologies). ..

Cell Culture:

Article Title: In vivo and in vitro effects of cord blood hematopoietic stem and progenitor cell (HSPC) expansion using valproic acid and/or nicotinamide.
Article Snippet: Background: High self-renewal capacity and most permissive nature of umbilical cord blood (CB) results with successful transplant outcomes but low hematopoietic stem and progenitor cell (HSPC) counts limits wider use.. In order to overcome this problem ex vivo expansion with small molecules such as Valproic acid (VPA) or Nicotinamide (NAM) have been shown to be effective.. To the best of our knowledge, the combinatory effects of VPA and NAM on HSPC expansion has not been studied earlier.

other:

Article Title: Anti-CLL-1 antibodies and methods of use
Article Snippet: In the following example, a human Colony-Forming Cell (CFU) assay, MethoCult, (StemCell Technologies, Vancouver, BC, Canada) was used to determine the effect of an anti-human CLL-1 TDB treatment on the ability of human bone marrow-derived HSCs to proliferate and differentiate in culture.



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